Construct: shRNA TRCN0000215994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172868.2-3533s1c1
DNA Barcode:
CCCTAATCTATTGGTCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Palm2 (242481)
Gene Description:
paralemmin 2
Transcript:
RefSeq NM_172868.2 (NON-CURRENT)
Match location:
Position 3533 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAATCTATTGGTCATTAT
Hairpin Sequence:
5'-CCGG-CCCTAATCTATTGGTCATTAT-CTCGAG-ATAATGACCAATAGATTAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAATCTATTGGTCATTATCTCGAGATAATGACCAATAGATTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAATCTATTGGTCATTATCTCGAGATAATGACCAATAGATTAGGG-3'

Other clones with same target sequence:

  1. TRCN0000246692