Construct: shRNA TRCN0000216043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177271.3-756s1c1
DNA Barcode:
CAGAACCACTAGATATCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Samd5 (320825)
Gene Description:
sterile alpha motif domain containing 5
Transcript:
RefSeq NM_177271.3 (CURRENT)
Match location:
Position 756 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACCACTAGATATCATTT
Hairpin Sequence:
5'-CCGG-CAGAACCACTAGATATCATTT-CTCGAG-AAATGATATCTAGTGGTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACCACTAGATATCATTTCTCGAGAAATGATATCTAGTGGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACCACTAGATATCATTTCTCGAGAAATGATATCTAGTGGTTCTG-3'

Other clones with same target sequence:

(none)