Construct: shRNA TRCN0000216053

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081249.1-3320s1c1
DNA Barcode:
CACTACTTATGTAGACATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vcan (13003)
Gene Description:
versican
Transcript:
RefSeq NM_001081249.1 (CURRENT)
Match location:
Position 3320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216053)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTACTTATGTAGACATATC
Hairpin Sequence:
5'-CCGG-CACTACTTATGTAGACATATC-CTCGAG-GATATGTCTACATAAGTAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTACTTATGTAGACATATCCTCGAGGATATGTCTACATAAGTAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTACTTATGTAGACATATCCTCGAGGATATGTCTACATAAGTAGTG-3'

Other clones with same target sequence:

(none)