Construct: shRNA TRCN0000216193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031998.2-2718s1c1
DNA Barcode:
CAACGTACACATAGCTTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep41 (83922)
Gene Description:
centrosomal protein 41
Transcript:
RefSeq NM_031998.2 (NON-CURRENT)
Match location:
Position 2718 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGTACACATAGCTTTAAT
Hairpin Sequence:
5'-CCGG-CAACGTACACATAGCTTTAAT-CTCGAG-ATTAAAGCTATGTGTACGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGTACACATAGCTTTAATCTCGAGATTAAAGCTATGTGTACGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGTACACATAGCTTTAATCTCGAGATTAAAGCTATGTGTACGTTG-3'

Other clones with same target sequence:

(none)