Construct: shRNA TRCN0000216282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173187.3-2630s1c1
DNA Barcode:
CAAGATGTGTCTACTATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2310035C23Rik (227446)
Gene Description:
RIKEN cDNA 2310035C23 gene
Transcript:
RefSeq NM_173187.3 (CURRENT)
Match location:
Position 2630 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGATGTGTCTACTATTATT
Hairpin Sequence:
5'-CCGG-CAAGATGTGTCTACTATTATT-CTCGAG-AATAATAGTAGACACATCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGATGTGTCTACTATTATTCTCGAGAATAATAGTAGACACATCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGATGTGTCTACTATTATTCTCGAGAATAATAGTAGACACATCTTG-3'

Other clones with same target sequence:

(none)