Construct: shRNA TRCN0000216321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033561.1-276s1c1
DNA Barcode:
GGGTTCTGCTATGTAGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif4h (22384)
Gene Description:
eukaryotic translation initiation factor 4H
Transcript:
RefSeq NM_033561.1 (NON-CURRENT)
Match location:
Position 276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTTCTGCTATGTAGAATTT
Hairpin Sequence:
5'-CCGG-GGGTTCTGCTATGTAGAATTT-CTCGAG-AAATTCTACATAGCAGAACCC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTTCTGCTATGTAGAATTTCTCGAGAAATTCTACATAGCAGAACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTTCTGCTATGTAGAATTTCTCGAGAAATTCTACATAGCAGAACCC-3'

Other clones with same target sequence:

(none)