Construct: shRNA TRCN0000216353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175175.3-869s1c1
DNA Barcode:
GTCGTTGAAGTCTCCTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekhf2 (71801)
Gene Description:
pleckstrin homology domain containing, family F (with FYVE domain) member 2
Transcript:
RefSeq NM_175175.3 (NON-CURRENT)
Match location:
Position 869 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGTTGAAGTCTCCTTTAAA
Hairpin Sequence:
5'-CCGG-GTCGTTGAAGTCTCCTTTAAA-CTCGAG-TTTAAAGGAGACTTCAACGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGTTGAAGTCTCCTTTAAACTCGAGTTTAAAGGAGACTTCAACGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGTTGAAGTCTCCTTTAAACTCGAGTTTAAAGGAGACTTCAACGAC-3'

Other clones with same target sequence:

(none)