Construct: shRNA TRCN0000216384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031811.1-3332s1c1
DNA Barcode:
GCAAAGCCATAAACGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnh8 (211468)
Gene Description:
potassium voltage-gated channel, subfamily H (eag-related), member 8
Transcript:
RefSeq NM_001031811.1 (NON-CURRENT)
Match location:
Position 3332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGCCATAAACGTATAAT
Hairpin Sequence:
5'-CCGG-GCAAAGCCATAAACGTATAAT-CTCGAG-ATTATACGTTTATGGCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGCCATAAACGTATAATCTCGAGATTATACGTTTATGGCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGCCATAAACGTATAATCTCGAGATTATACGTTTATGGCTTTGC-3'

Other clones with same target sequence:

(none)