Construct: shRNA TRCN0000216402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173038.3-4611s1c1
DNA Barcode:
GTTTGTATGTACGATAATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbcel (272589)
Gene Description:
tubulin folding cofactor E-like
Transcript:
RefSeq NM_173038.3 (CURRENT)
Match location:
Position 4611 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGTATGTACGATAATTTC
Hairpin Sequence:
5'-CCGG-GTTTGTATGTACGATAATTTC-CTCGAG-GAAATTATCGTACATACAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGTATGTACGATAATTTCCTCGAGGAAATTATCGTACATACAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGTATGTACGATAATTTCCTCGAGGAAATTATCGTACATACAAAC-3'

Other clones with same target sequence:

(none)