Construct: shRNA TRCN0000216440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145381.2-1211s1c1
DNA Barcode:
GAACAATTCAAACCAGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lactb2 (212442)
Gene Description:
lactamase, beta 2
Transcript:
RefSeq NM_145381.2 (CURRENT)
Match location:
Position 1211 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAATTCAAACCAGTTAAT
Hairpin Sequence:
5'-CCGG-GAACAATTCAAACCAGTTAAT-CTCGAG-ATTAACTGGTTTGAATTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAATTCAAACCAGTTAATCTCGAGATTAACTGGTTTGAATTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAATTCAAACCAGTTAATCTCGAGATTAACTGGTTTGAATTGTTC-3'

Other clones with same target sequence:

(none)