Construct: shRNA TRCN0000216469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033350.2-1026s1c1
DNA Barcode:
GATAATGTTCTTGCATCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bank1 (242248)
Gene Description:
B cell scaffold protein with ankyrin repeats 1
Transcript:
RefSeq NM_001033350.2 (NON-CURRENT)
Match location:
Position 1026 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAATGTTCTTGCATCTATA
Hairpin Sequence:
5'-CCGG-GATAATGTTCTTGCATCTATA-CTCGAG-TATAGATGCAAGAACATTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAATGTTCTTGCATCTATACTCGAGTATAGATGCAAGAACATTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAATGTTCTTGCATCTATACTCGAGTATAGATGCAAGAACATTATC-3'

Other clones with same target sequence:

  1. TRCN0000242020