Construct: shRNA TRCN0000216471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009551.4-1455s1c1
DNA Barcode:
GGAATGTAATCAAGGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfand5 (22682)
Gene Description:
zinc finger, AN1-type domain 5
Transcript:
RefSeq NM_009551.4 (NON-CURRENT)
Match location:
Position 1455 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAATGTAATCAAGGTATAAT
Hairpin Sequence:
5'-CCGG-GGAATGTAATCAAGGTATAAT-CTCGAG-ATTATACCTTGATTACATTCC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAATGTAATCAAGGTATAATCTCGAGATTATACCTTGATTACATTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAATGTAATCAAGGTATAATCTCGAGATTATACCTTGATTACATTCC-3'

Other clones with same target sequence:

  1. TRCN0000240691