Construct: shRNA TRCN0000216477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026367.3-1131s1c1
DNA Barcode:
GAACGCCTTCAAAGAATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpatch2 (67769)
Gene Description:
G patch domain containing 2
Transcript:
RefSeq NM_026367.3 (NON-CURRENT)
Match location:
Position 1131 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGCCTTCAAAGAATATTA
Hairpin Sequence:
5'-CCGG-GAACGCCTTCAAAGAATATTA-CTCGAG-TAATATTCTTTGAAGGCGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGCCTTCAAAGAATATTACTCGAGTAATATTCTTTGAAGGCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGCCTTCAAAGAATATTACTCGAGTAATATTCTTTGAAGGCGTTC-3'

Other clones with same target sequence:

  1. TRCN0000258031