Construct: shRNA TRCN0000216501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026353.3-794s1c1
DNA Barcode:
CCTTTCCAAATAGATCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc48a1 (67739)
Gene Description:
solute carrier family 48 (heme transporter), member 1
Transcript:
RefSeq NM_026353.3 (NON-CURRENT)
Match location:
Position 794 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCCAAATAGATCTATTT
Hairpin Sequence:
5'-CCGG-CCTTTCCAAATAGATCTATTT-CTCGAG-AAATAGATCTATTTGGAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCCAAATAGATCTATTTCTCGAGAAATAGATCTATTTGGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCCAAATAGATCTATTTCTCGAGAAATAGATCTATTTGGAAAGG-3'

Other clones with same target sequence:

(none)