Construct: shRNA TRCN0000216551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026470.3-2280s1c1
DNA Barcode:
GTCATTCATGTAGCTAATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata6 (67946)
Gene Description:
spermatogenesis associated 6
Transcript:
RefSeq NM_026470.3 (CURRENT)
Match location:
Position 2280 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATTCATGTAGCTAATATT
Hairpin Sequence:
5'-CCGG-GTCATTCATGTAGCTAATATT-CTCGAG-AATATTAGCTACATGAATGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATTCATGTAGCTAATATTCTCGAGAATATTAGCTACATGAATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATTCATGTAGCTAATATTCTCGAGAATATTAGCTACATGAATGAC-3'

Other clones with same target sequence:

  1. TRCN0000248455