Construct: shRNA TRCN0000216571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177784.3-1076s1c1
DNA Barcode:
GAGCAGAAATACCAGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhl23 (277396)
Gene Description:
kelch-like 23
Transcript:
RefSeq NM_177784.3 (NON-CURRENT)
Match location:
Position 1076 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGAAATACCAGATTATA
Hairpin Sequence:
5'-CCGG-GAGCAGAAATACCAGATTATA-CTCGAG-TATAATCTGGTATTTCTGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGAAATACCAGATTATACTCGAGTATAATCTGGTATTTCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGAAATACCAGATTATACTCGAGTATAATCTGGTATTTCTGCTC-3'

Other clones with same target sequence:

(none)