Construct: shRNA TRCN0000216572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178626.2-2027s1c1
DNA Barcode:
GAAAGCATTTCTCCTATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc42se2 (72729)
Gene Description:
CDC42 small effector 2
Transcript:
RefSeq NM_178626.2 (NON-CURRENT)
Match location:
Position 2027 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGCATTTCTCCTATATTT
Hairpin Sequence:
5'-CCGG-GAAAGCATTTCTCCTATATTT-CTCGAG-AAATATAGGAGAAATGCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGCATTTCTCCTATATTTCTCGAGAAATATAGGAGAAATGCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGCATTTCTCCTATATTTCTCGAGAAATATAGGAGAAATGCTTTC-3'

Other clones with same target sequence:

(none)