Construct: shRNA TRCN0000216605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027495.4-1715s1c1
DNA Barcode:
GAAATTACCATCCCAATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem144 (70652)
Gene Description:
transmembrane protein 144
Transcript:
RefSeq NM_027495.4 (CURRENT)
Match location:
Position 1715 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATTACCATCCCAATTTAT
Hairpin Sequence:
5'-CCGG-GAAATTACCATCCCAATTTAT-CTCGAG-ATAAATTGGGATGGTAATTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATTACCATCCCAATTTATCTCGAGATAAATTGGGATGGTAATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATTACCATCCCAATTTATCTCGAGATAAATTGGGATGGTAATTTC-3'

Other clones with same target sequence:

(none)