Construct: shRNA TRCN0000216611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139140.1-546s1c1
DNA Barcode:
GCTCAAAGAATGGATAGTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spats2 (72572)
Gene Description:
spermatogenesis associated, serine-rich 2
Transcript:
RefSeq NM_139140.1 (CURRENT)
Match location:
Position 546 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAAAGAATGGATAGTAAC
Hairpin Sequence:
5'-CCGG-GCTCAAAGAATGGATAGTAAC-CTCGAG-GTTACTATCCATTCTTTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAAAGAATGGATAGTAACCTCGAGGTTACTATCCATTCTTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAAAGAATGGATAGTAACCTCGAGGTTACTATCCATTCTTTGAGC-3'

Other clones with same target sequence:

(none)