Construct: shRNA TRCN0000216632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024952.2-8198s1c1
DNA Barcode:
CTTAGTACCTGTTCGACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rc3h1 (381305)
Gene Description:
RING CCCH (C3H) domains 1
Transcript:
RefSeq NM_001024952.2 (CURRENT)
Match location:
Position 8198 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTAGTACCTGTTCGACTTAA
Hairpin Sequence:
5'-CCGG-CTTAGTACCTGTTCGACTTAA-CTCGAG-TTAAGTCGAACAGGTACTAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTAGTACCTGTTCGACTTAACTCGAGTTAAGTCGAACAGGTACTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTAGTACCTGTTCGACTTAACTCGAGTTAAGTCGAACAGGTACTAAG-3'

Other clones with same target sequence:

(none)