Construct: shRNA TRCN0000216635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011734.3-2375s1c1
DNA Barcode:
CAACTAAGCCTAAGCCTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Siae (22619)
Gene Description:
sialic acid acetylesterase
Transcript:
RefSeq NM_011734.3 (CURRENT)
Match location:
Position 2375 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTAAGCCTAAGCCTAATT
Hairpin Sequence:
5'-CCGG-CAACTAAGCCTAAGCCTAATT-CTCGAG-AATTAGGCTTAGGCTTAGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTAAGCCTAAGCCTAATTCTCGAGAATTAGGCTTAGGCTTAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTAAGCCTAAGCCTAATTCTCGAGAATTAGGCTTAGGCTTAGTTG-3'

Other clones with same target sequence:

(none)