Construct: shRNA TRCN0000216677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178115.3-2567s1c1
DNA Barcode:
CTTGCAATTCAGCGACTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edrf1 (214764)
Gene Description:
erythroid differentiation regulatory factor 1
Transcript:
RefSeq NM_178115.3 (NON-CURRENT)
Match location:
Position 2567 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGCAATTCAGCGACTTAAA
Hairpin Sequence:
5'-CCGG-CTTGCAATTCAGCGACTTAAA-CTCGAG-TTTAAGTCGCTGAATTGCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGCAATTCAGCGACTTAAACTCGAGTTTAAGTCGCTGAATTGCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGCAATTCAGCGACTTAAACTCGAGTTTAAGTCGCTGAATTGCAAG-3'

Other clones with same target sequence:

(none)