Construct: shRNA TRCN0000216793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194344.2-3633s1c1
DNA Barcode:
GCAGGGAAGATCTACTATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3tc1 (231147)
Gene Description:
SH3 domain and tetratricopeptide repeats 1
Transcript:
RefSeq NM_194344.2 (CURRENT)
Match location:
Position 3633 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGAAGATCTACTATATC
Hairpin Sequence:
5'-CCGG-GCAGGGAAGATCTACTATATC-CTCGAG-GATATAGTAGATCTTCCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGAAGATCTACTATATCCTCGAGGATATAGTAGATCTTCCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGAAGATCTACTATATCCTCGAGGATATAGTAGATCTTCCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000265272