Construct: shRNA TRCN0000216795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033812.1-1946s1c1
DNA Barcode:
GATTGCAGGCTTTAGTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
F830208F22Rik (619308)
Gene Description:
RIKEN cDNA F830208F22 gene
Transcript:
RefSeq NM_001033812.1 (NON-CURRENT)
Match location:
Position 1946 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGCAGGCTTTAGTCTAAA
Hairpin Sequence:
5'-CCGG-GATTGCAGGCTTTAGTCTAAA-CTCGAG-TTTAGACTAAAGCCTGCAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGCAGGCTTTAGTCTAAACTCGAGTTTAGACTAAAGCCTGCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGCAGGCTTTAGTCTAAACTCGAGTTTAGACTAAAGCCTGCAATC-3'

Other clones with same target sequence:

(none)