Construct: shRNA TRCN0000216814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152815.1-830s1c1
DNA Barcode:
GATATTCTTGCCTGGCCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lins1 (72635)
Gene Description:
lines homolog 1
Transcript:
RefSeq NM_152815.1 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATATTCTTGCCTGGCCTATT
Hairpin Sequence:
5'-CCGG-GATATTCTTGCCTGGCCTATT-CTCGAG-AATAGGCCAGGCAAGAATATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATATTCTTGCCTGGCCTATTCTCGAGAATAGGCCAGGCAAGAATATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATATTCTTGCCTGGCCTATTCTCGAGAATAGGCCAGGCAAGAATATC-3'

Other clones with same target sequence:

(none)