Construct: shRNA TRCN0000216841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053204.2-3133s1c1
DNA Barcode:
GATAACTGCAGAGCGTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erc1 (111173)
Gene Description:
ELKS/RAB6-interacting/CAST family member 1
Transcript:
RefSeq NM_053204.2 (CURRENT)
Match location:
Position 3133 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAACTGCAGAGCGTGAAAT
Hairpin Sequence:
5'-CCGG-GATAACTGCAGAGCGTGAAAT-CTCGAG-ATTTCACGCTCTGCAGTTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAACTGCAGAGCGTGAAATCTCGAGATTTCACGCTCTGCAGTTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAACTGCAGAGCGTGAAATCTCGAGATTTCACGCTCTGCAGTTATC-3'

Other clones with same target sequence:

(none)