Construct: shRNA TRCN0000216948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028651.2-2003s1c1
DNA Barcode:
CGTGGAACAACATGATCATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmtc4 (70551)
Gene Description:
transmembrane and tetratricopeptide repeat containing 4
Transcript:
RefSeq NM_028651.2 (CURRENT)
Match location:
Position 2003 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGAACAACATGATCATAC
Hairpin Sequence:
5'-CCGG-CGTGGAACAACATGATCATAC-CTCGAG-GTATGATCATGTTGTTCCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGAACAACATGATCATACCTCGAGGTATGATCATGTTGTTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGAACAACATGATCATACCTCGAGGTATGATCATGTTGTTCCACG-3'

Other clones with same target sequence:

(none)