Construct: shRNA TRCN0000216949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028709.1-1946s1c1
DNA Barcode:
CGTGGATGTCACAATTGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Btbd11 (74007)
Gene Description:
BTB (POZ) domain containing 11
Transcript:
RefSeq NM_028709.1 (NON-CURRENT)
Match location:
Position 1946 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGATGTCACAATTGATAT
Hairpin Sequence:
5'-CCGG-CGTGGATGTCACAATTGATAT-CTCGAG-ATATCAATTGTGACATCCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGATGTCACAATTGATATCTCGAGATATCAATTGTGACATCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGATGTCACAATTGATATCTCGAGATATCAATTGTGACATCCACG-3'

Other clones with same target sequence:

  1. TRCN0000241367