Construct: shRNA TRCN0000216999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178671.3-925s1c1
DNA Barcode:
CCCTTTACCAAGAGGATTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubxn10 (212190)
Gene Description:
UBX domain protein 10
Transcript:
RefSeq NM_178671.3 (NON-CURRENT)
Match location:
Position 925 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTACCAAGAGGATTCAA
Hairpin Sequence:
5'-CCGG-CCCTTTACCAAGAGGATTCAA-CTCGAG-TTGAATCCTCTTGGTAAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTACCAAGAGGATTCAACTCGAGTTGAATCCTCTTGGTAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTACCAAGAGGATTCAACTCGAGTTGAATCCTCTTGGTAAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000251002