Construct: shRNA TRCN0000217013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025942.2-833s1c1
DNA Barcode:
CAATGGTCTACTTGGTGAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ola1 (67059)
Gene Description:
Obg-like ATPase 1
Transcript:
RefSeq NM_025942.2 (CURRENT)
Match location:
Position 833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGGTCTACTTGGTGAATC
Hairpin Sequence:
5'-CCGG-CAATGGTCTACTTGGTGAATC-CTCGAG-GATTCACCAAGTAGACCATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGGTCTACTTGGTGAATCCTCGAGGATTCACCAAGTAGACCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGGTCTACTTGGTGAATCCTCGAGGATTCACCAAGTAGACCATTG-3'

Other clones with same target sequence:

(none)