Construct: shRNA TRCN0000217046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001052081.2-795s1c1
DNA Barcode:
CCAGGATGACCTGATAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Catsperd (106757)
Gene Description:
cation channel sperm associated auxiliary subunit delta
Transcript:
RefSeq XM_001052081.2 (NON-CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGATGACCTGATAGTATT
Hairpin Sequence:
5'-CCGG-CCAGGATGACCTGATAGTATT-CTCGAG-AATACTATCAGGTCATCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGATGACCTGATAGTATTCTCGAGAATACTATCAGGTCATCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGATGACCTGATAGTATTCTCGAGAATACTATCAGGTCATCCTGG-3'

Other clones with same target sequence:

(none)