Construct: shRNA TRCN0000217049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024927.2-2728s1c1
DNA Barcode:
CTATGGCTCCACGAAAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pitpnm3 (327958)
Gene Description:
PITPNM family member 3
Transcript:
RefSeq NM_001024927.2 (CURRENT)
Match location:
Position 2728 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGGCTCCACGAAAGATAT
Hairpin Sequence:
5'-CCGG-CTATGGCTCCACGAAAGATAT-CTCGAG-ATATCTTTCGTGGAGCCATAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGGCTCCACGAAAGATATCTCGAGATATCTTTCGTGGAGCCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGGCTCCACGAAAGATATCTCGAGATATCTTTCGTGGAGCCATAG-3'

Other clones with same target sequence:

(none)