Construct: shRNA TRCN0000217068

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026083.2-3483s1c1
DNA Barcode:
CATGAGGATAGTCAGGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc3h13 (67302)
Gene Description:
zinc finger CCCH type containing 13
Transcript:
RefSeq NM_026083.2 (CURRENT)
Match location:
Position 3483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217068)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAGGATAGTCAGGTATTT
Hairpin Sequence:
5'-CCGG-CATGAGGATAGTCAGGTATTT-CTCGAG-AAATACCTGACTATCCTCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAGGATAGTCAGGTATTTCTCGAGAAATACCTGACTATCCTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAGGATAGTCAGGTATTTCTCGAGAAATACCTGACTATCCTCATG-3'

Other clones with same target sequence:

(none)