Construct: shRNA TRCN0000217142

Construct Description:

• Construct Type: shRNA
• Other Identifiers: NM_027283.1-130s1c1
• DNA Barcode: CAGCATCGAGCAGAAGTATAA

Original Target:

• Taxon: Mus musculus (mouse)
• Gene: Spaca9 (69987)
• Gene Description: sperm acrosome associated 9
• Transcript: RefSeq NM_027283.1 (CURRENT)
• Match location: Position 130 (CDS)

Vector Information:

• Vector Backbone: pLKO.1
• Pol II Cassette 1: PGK-PuroR
• Pol II Cassette 2: n/a
• Pol III Promoter: constitutive hU6
• Pol III Insert: (TRCN0000217142)
• Selection Marker: PuroR
• Visible Reporter: n/a

Current transcripts matched by this shRNA:

	Taxon	Gene	Symbol	Description	Transcript	SDR Match %	Region	Start Pos.	Intrinsic Score	Adjusted Score
1	mouse	69987	Spaca9	sperm acrosome associated 9	NM_027283.1	100%	CDS	130	13.200	18.480
2	human	11092	SPACA9	sperm acrosome associated 9	NM_001316897.2	95%	CDS	181
3	human	11092	SPACA9	sperm acrosome associated 9	NM_001316898.2	95%	CDS	157
4	human	11092	SPACA9	sperm acrosome associated 9	NM_001316900.2	95%	CDS	181
5	human	11092	SPACA9	sperm acrosome associated 9	NM_018956.5	95%	CDS	181
6	human	11092	SPACA9	sperm acrosome associated 9	NR_133631.2	95%	3UTR	157
7	human	11092	SPACA9	sperm acrosome associated 9	XM_017014231.1	95%	CDS	184
8	human	11092	SPACA9	sperm acrosome associated 9	XM_024447396.1	95%	CDS	262
9	human	11092	SPACA9	sperm acrosome associated 9	XM_024447397.1	95%	CDS	181
10	human	102724637	LOC102724637	uncharacterized LOC102724637	XR_948559.2	89%	3UTR	2732

Sequence Information

• Target Sequence: CAGCATCGAGCAGAAGTATAA
• Hairpin Sequence: 5'-CCGG-CAGCATCGAGCAGAAGTATAA-CTCGAG-TTATACTTCTGCTCGATGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

• Forward sequence: 5'-CCGGCAGCATCGAGCAGAAGTATAACTCGAGTTATACTTCTGCTCGATGCTGTTTTTG-3'
• Reverse sequence: 5'-AATTCAAAAACAGCATCGAGCAGAAGTATAACTCGAGTTATACTTCTGCTCGATGCTG-3'

Other clones with same target sequence:

(none)