Construct: shRNA TRCN0000217159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025574.3-3216s1c1
DNA Barcode:
CATCTAGCTACCCACGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pyurf (66459)
Gene Description:
Pigy upstream reading frame
Transcript:
RefSeq NM_025574.3 (CURRENT)
Match location:
Position 3216 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTAGCTACCCACGTTTAT
Hairpin Sequence:
5'-CCGG-CATCTAGCTACCCACGTTTAT-CTCGAG-ATAAACGTGGGTAGCTAGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTAGCTACCCACGTTTATCTCGAGATAAACGTGGGTAGCTAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTAGCTACCCACGTTTATCTCGAGATAAACGTGGGTAGCTAGATG-3'

Other clones with same target sequence:

(none)