Construct: shRNA TRCN0000217197

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146047.1-90s1c1
DNA Barcode:
CAAACTGCAGCTGAGTGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clptm1l (218335)
Gene Description:
CLPTM1-like
Transcript:
RefSeq NM_146047.1 (NON-CURRENT)
Match location:
Position 90 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217197)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACTGCAGCTGAGTGTTTA
Hairpin Sequence:
5'-CCGG-CAAACTGCAGCTGAGTGTTTA-CTCGAG-TAAACACTCAGCTGCAGTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACTGCAGCTGAGTGTTTACTCGAGTAAACACTCAGCTGCAGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACTGCAGCTGAGTGTTTACTCGAGTAAACACTCAGCTGCAGTTTG-3'

Other clones with same target sequence:

(none)