Construct: shRNA TRCN0000217251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145939.1-522s1c1
DNA Barcode:
CTTCAATGACCCAGTAGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alg3 (208624)
Gene Description:
asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase)
Transcript:
RefSeq NM_145939.1 (NON-CURRENT)
Match location:
Position 522 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAATGACCCAGTAGCTAT
Hairpin Sequence:
5'-CCGG-CTTCAATGACCCAGTAGCTAT-CTCGAG-ATAGCTACTGGGTCATTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAATGACCCAGTAGCTATCTCGAGATAGCTACTGGGTCATTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAATGACCCAGTAGCTATCTCGAGATAGCTACTGGGTCATTGAAG-3'

Other clones with same target sequence:

(none)