Construct: shRNA TRCN0000217274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028840.2-648s1c1
DNA Barcode:
CATAGATGGTCTCGATGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc1 (74252)
Gene Description:
armadillo repeat containing 1
Transcript:
RefSeq NM_028840.2 (CURRENT)
Match location:
Position 648 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGATGGTCTCGATGATAC
Hairpin Sequence:
5'-CCGG-CATAGATGGTCTCGATGATAC-CTCGAG-GTATCATCGAGACCATCTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGATGGTCTCGATGATACCTCGAGGTATCATCGAGACCATCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGATGGTCTCGATGATACCTCGAGGTATCATCGAGACCATCTATG-3'

Other clones with same target sequence:

  1. TRCN0000249032