Construct: shRNA TRCN0000217293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026412.2-447s1c1
DNA Barcode:
GCGGATTTGCTTAGACATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Knstrn (51944)
Gene Description:
kinetochore-localized astrin/SPAG5 binding
Transcript:
RefSeq NM_026412.2 (NON-CURRENT)
Match location:
Position 447 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGATTTGCTTAGACATCTT
Hairpin Sequence:
5'-CCGG-GCGGATTTGCTTAGACATCTT-CTCGAG-AAGATGTCTAAGCAAATCCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGATTTGCTTAGACATCTTCTCGAGAAGATGTCTAAGCAAATCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGATTTGCTTAGACATCTTCTCGAGAAGATGTCTAAGCAAATCCGC-3'

Other clones with same target sequence:

(none)