Construct: shRNA TRCN0000217318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002268.3-3591s1c1
DNA Barcode:
GAAACAGCCACTCGGATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrg6 (215798)
Gene Description:
adhesion G protein-coupled receptor G6
Transcript:
RefSeq NM_001002268.3 (CURRENT)
Match location:
Position 3591 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACAGCCACTCGGATAATT
Hairpin Sequence:
5'-CCGG-GAAACAGCCACTCGGATAATT-CTCGAG-AATTATCCGAGTGGCTGTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACAGCCACTCGGATAATTCTCGAGAATTATCCGAGTGGCTGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACAGCCACTCGGATAATTCTCGAGAATTATCCGAGTGGCTGTTTC-3'

Other clones with same target sequence:

(none)