Construct: shRNA TRCN0000217382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026197.3-1176s1c1
DNA Barcode:
GATCTGAAGGTGCAACATAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl16 (67493)
Gene Description:
methyltransferase like 16
Transcript:
RefSeq NM_026197.3 (CURRENT)
Match location:
Position 1176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTGAAGGTGCAACATAAG
Hairpin Sequence:
5'-CCGG-GATCTGAAGGTGCAACATAAG-CTCGAG-CTTATGTTGCACCTTCAGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTGAAGGTGCAACATAAGCTCGAGCTTATGTTGCACCTTCAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTGAAGGTGCAACATAAGCTCGAGCTTATGTTGCACCTTCAGATC-3'

Other clones with same target sequence:

(none)