Construct: shRNA TRCN0000217386
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_029166.1-4652s1c1
- DNA Barcode:
- GAAGGATGTAACCAGTTAGTG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Uhrf1bp1l (75089)
- Gene Description:
- UHRF1 (ICBP90) binding protein 1-like
- Transcript:
- RefSeq NM_029166.1 (NON-CURRENT)
- Match location:
- Position 4652 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000217386)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGGATGTAACCAGTTAGTG
- Hairpin Sequence:
-
5'-CCGG-GAAGGATGTAACCAGTTAGTG-CTCGAG-CACTAACTGGTTACATCCTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGGATGTAACCAGTTAGTGCTCGAGCACTAACTGGTTACATCCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGGATGTAACCAGTTAGTGCTCGAGCACTAACTGGTTACATCCTTC-3'
Other clones with same target sequence:
(none)
