Construct: shRNA TRCN0000217449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178764.3-4785s1c1
DNA Barcode:
GATCCAACAAAGTACGTTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam168a (319604)
Gene Description:
family with sequence similarity 168, member A
Transcript:
RefSeq NM_178764.3 (CURRENT)
Match location:
Position 4785 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAACAAAGTACGTTAAC
Hairpin Sequence:
5'-CCGG-GATCCAACAAAGTACGTTAAC-CTCGAG-GTTAACGTACTTTGTTGGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAACAAAGTACGTTAACCTCGAGGTTAACGTACTTTGTTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAACAAAGTACGTTAACCTCGAGGTTAACGTACTTTGTTGGATC-3'

Other clones with same target sequence:

(none)