Construct: shRNA TRCN0000217521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175541.2-1101s1c1
DNA Barcode:
GATTTGTGACTACCGAGTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mum1l1 (245631)
Gene Description:
melanoma associated antigen (mutated) 1-like 1
Transcript:
RefSeq NM_175541.2 (NON-CURRENT)
Match location:
Position 1101 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTGTGACTACCGAGTTAG
Hairpin Sequence:
5'-CCGG-GATTTGTGACTACCGAGTTAG-CTCGAG-CTAACTCGGTAGTCACAAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTGTGACTACCGAGTTAGCTCGAGCTAACTCGGTAGTCACAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTGTGACTACCGAGTTAGCTCGAGCTAACTCGGTAGTCACAAATC-3'

Other clones with same target sequence:

(none)