Construct: shRNA TRCN0000217558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027332.3-1687s1c1
DNA Barcode:
GCAATTAGGTTGTCGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpf1 (70285)
Gene Description:
ribosome production factor 1 homolog
Transcript:
RefSeq NM_027332.3 (CURRENT)
Match location:
Position 1687 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATTAGGTTGTCGTCATTT
Hairpin Sequence:
5'-CCGG-GCAATTAGGTTGTCGTCATTT-CTCGAG-AAATGACGACAACCTAATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATTAGGTTGTCGTCATTTCTCGAGAAATGACGACAACCTAATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATTAGGTTGTCGTCATTTCTCGAGAAATGACGACAACCTAATTGC-3'

Other clones with same target sequence:

(none)