Construct: shRNA TRCN0000217585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026797.2-1106s1c1
DNA Barcode:
GGGAACAGGATATGGTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dbndd2 (52840)
Gene Description:
dysbindin (dystrobrevin binding protein 1) domain containing 2
Transcript:
RefSeq NM_026797.2 (CURRENT)
Match location:
Position 1106 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAACAGGATATGGTATAAA
Hairpin Sequence:
5'-CCGG-GGGAACAGGATATGGTATAAA-CTCGAG-TTTATACCATATCCTGTTCCC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAACAGGATATGGTATAAACTCGAGTTTATACCATATCCTGTTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAACAGGATATGGTATAAACTCGAGTTTATACCATATCCTGTTCCC-3'

Other clones with same target sequence:

(none)