Construct: shRNA TRCN0000217626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198014.2-2125s1c1
DNA Barcode:
GAGATTGAGGCGTATCAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slain1 (105439)
Gene Description:
SLAIN motif family, member 1
Transcript:
RefSeq NM_198014.2 (CURRENT)
Match location:
Position 2125 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATTGAGGCGTATCAATAA
Hairpin Sequence:
5'-CCGG-GAGATTGAGGCGTATCAATAA-CTCGAG-TTATTGATACGCCTCAATCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATTGAGGCGTATCAATAACTCGAGTTATTGATACGCCTCAATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATTGAGGCGTATCAATAACTCGAGTTATTGATACGCCTCAATCTC-3'

Other clones with same target sequence:

(none)