Construct: shRNA TRCN0000217647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029868.2-2326s1c1
DNA Barcode:
GAAGCAGAGCACAGGTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpbp1l1 (77110)
Gene Description:
GC-rich promoter binding protein 1-like 1
Transcript:
RefSeq NM_029868.2 (CURRENT)
Match location:
Position 2326 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCAGAGCACAGGTTATTA
Hairpin Sequence:
5'-CCGG-GAAGCAGAGCACAGGTTATTA-CTCGAG-TAATAACCTGTGCTCTGCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCAGAGCACAGGTTATTACTCGAGTAATAACCTGTGCTCTGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCAGAGCACAGGTTATTACTCGAGTAATAACCTGTGCTCTGCTTC-3'

Other clones with same target sequence:

  1. TRCN0000246905