Construct: shRNA TRCN0000217665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021493.2-1758s1c1
DNA Barcode:
GATGCAACCTTCAGTGATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap23 (58996)
Gene Description:
Rho GTPase activating protein 23
Transcript:
RefSeq NM_021493.2 (CURRENT)
Match location:
Position 1758 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCAACCTTCAGTGATATC
Hairpin Sequence:
5'-CCGG-GATGCAACCTTCAGTGATATC-CTCGAG-GATATCACTGAAGGTTGCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCAACCTTCAGTGATATCCTCGAGGATATCACTGAAGGTTGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCAACCTTCAGTGATATCCTCGAGGATATCACTGAAGGTTGCATC-3'

Other clones with same target sequence:

(none)