Construct: shRNA TRCN0000217675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031382.2-100s1c1
DNA Barcode:
GGTAGAGCATCAGTATCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tex16 (83556)
Gene Description:
testis expressed gene 16
Transcript:
RefSeq NM_031382.2 (CURRENT)
Match location:
Position 100 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTAGAGCATCAGTATCTAAA
Hairpin Sequence:
5'-CCGG-GGTAGAGCATCAGTATCTAAA-CTCGAG-TTTAGATACTGATGCTCTACC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTAGAGCATCAGTATCTAAACTCGAGTTTAGATACTGATGCTCTACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTAGAGCATCAGTATCTAAACTCGAGTTTAGATACTGATGCTCTACC-3'

Other clones with same target sequence:

  1. TRCN0000257754